Researcher Information

MIZUSHIMA Shusei

Assistant Professor

Understanidng of avian spesific polyspermic fertilization system

Department of Biological Sciences, Reproductive and Developmental Biology

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Theme

Molecular Mechanisms of fertilization and early development and establishment of novel gene modification methods in birds

FieldAvian Reproductive Biology
KeywordBird, Polyspermic fertilization, Egg activation, Germ cell, Cleavage, In vitro fertilization, Genome editing

Introduction of Research

Birds exhibit physiologically polyspermic fertilization, in which the entry of several sperm into one egg is permitted. We have previously established avian intracytoplasmic sperm injection (ICSI) method and this technique have revealed that a single sperm is insufficient for triggering fertilization and subsequent early embryo development. In order to understand more comprehensive avian polyspermic fertilization, we try to elucidate the molecular mechanism which regulates sperm selection for zygotic formation, now (Figure 1 Movement and degradation of sperm nuclei in germinal disc of avian egg).

We also try to establish a new method of avian transgenesis and gene editing by in vitro fertilization technique (Figure 2 Avian specific in vitro fertilization system), because a progress in gene modification in birds has been hampered due to the lack of traditional strategies such as pronuclear injection of DNA, sperm vector and somatic nuclear transfer. Establishment of this new avian technologies will aid our understanding the mechanism of avian fertilization and sex differentiation of germ cell and so on, as well as assisting in the production low allergenic egg via the disruption of allergen-related genes.

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Figure 2 Avian specific in vitro fertilization system
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Figure 2 Avian specific in vitro fertilization system

Representative Achievements

Fertilization 2: Polyspermic fertilization II, S. Mizushima, Adv. Exp. Med. Biol., 2017, 1001, 105-123.
Handling of gamates for in vitro insemination in birds. S. Mizushima, M. Matsuzaki, T. Sasanami, Method. Mol. Biol., 2017, 1650, 243-257.
Lactic acid is a sperm motility inactivation factor in the sperm storage tubules of the domestic bird, M. Matsuzaki, S. Mizushima, G. Hiyama, N. Hirohashi, K. Shiba, K. Inaba, T. Suzuki, H. Dohra, T. Ohnishi, Y. Sato, T. Kohsaka, Y. Ichikawa, Y. Atsumi, T. Yoshimura, T. Sasanami, Sci. Rep., 2015, 5, 17643.
The birth of quail chicks after intracytoplasmic sperm injection, S. Mizushima, G. Hiyama, K. Shiba, K. Inaba, H. Dohra, T. Ono, K. Shimada, T. Sasanami, Development, 2014, 141, 3799-3806.
Novel method of gene transfer in birds: intracytoplasmic sperm injection for green fluorescent protein expression in quail blastoderms, S. Mizushima, T. Ono, Y. Atsumi, A. Tsukada, N. Saito, T. Sasanami, M. Okabe, K. Shimada, Biol. Reprod., 2010, 83, 965-969.
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Academic background2002 Bachelor of Animal Science and Technology, School of Veterinary Medicine, Azabu University
2005 Master of Bioagricultural Science, Graduate School of Bioagricultural Sciences, Nagoya University
2008 Doctor of Bioagricultural Science (Ph.D.), Graduate School of Bioagricultural Sciences, Nagoya University
2008-2011 Research Associate, Faculty of Human Sciences, Waseda University
2011-2014 Research Fellowship for Young Scientists (PD), Departement of Agriculture, Shizuoka University
2015-2016 Assistant Professor, Graduate School of Science and Engineering for Research, University of Toyama
2017- Assistant Professor, Faculty of Science, Hokkaido University
2015- Visiting Associate Professor, Departement of Agriculture, Shizuoka University
Affiliated academic societyJapan Poultry Science Association, World Poultry Science Association, Japanese Society of Animal Sciences, Zoological Science of Japan
Room addressScience Buiding 5 11-03

Department of Biological Sciences, Reproductive and Developmental Biology

MIZUSHIMA Shusei

Assistant Professor

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What is your dream that you want to achieve through your research?

I want to restore extinct avian species. The number of extinct and endangered species is rapidly increasing due to the environmental destruction of wildlife habitats and the detrimental effects of environmental pollutants. In particular, highly pathogenic avian influenza in recent years has become a worldwide threat. At present, however, it is difficult to preserve avian germ cells (sperm and eggs) for a long period, and in vitro fertilization techniques (one of artificial fertilization methods) are not well established. Currently, I am conducting research aimed at developing technologies that can restore quail and chicken individuals in vitro by referring to mammalian fertility treatments and somatic cell nuclear transfer (clone) techniques.

Quail chicks born by in vitro fertilization
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What is the research theme that you are currently focusing on?

Several animals such as birds, reptile and most urodele amphibians exhibit physiologically polyspermic fertilization, in which the entry of several sperm into one egg is permitted. Especially, the avian egg receives a markedly higher number of sperm than other polyspermic species. However, cleavage proceeds even when the sperm number incorporated into egg cytoplasm is low, but no germ cells are formed. I am currently studying the molecular mechanisms of early germ cell development.

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Please tell us about yourself; things you are good at, your favorites, hobbies, and daily routines.

My hobby is touring round historic sites. In particular, I learn about the anecdotes of Samurai (Sengoku Warlord) who ran through the Sengoku period based on materials available at ancient battlefield sites, temples and shrines. Also, Hokkaido is a region where nature is abundant, so I enjoy its unique landscape and mountain climbing. It’s very refreshing. I want to climb all 100 famous mountains in Hokkaido.